EMBL Outstation - The European Bioinformatics Institute

                        EMBL Nucleotide Sequence Database

                                Release Notes 
                            Release 64 Sep 2000

                                EMBL Outstation
                       European  Bioinformatics Institute
                          Wellcome Trust Genome Campus
                               Cambridge CB10 1SD
                                 United Kingdom

                           Telephone: +44-1223-494400
                           Telefax  : +44-1223-494468

                       Electronic mail: datalib@ebi.ac.uk
                           URL: http://www.ebi.ac.uk


   * 1 RELEASE 64
        o 1.1  Nine Billion Nucleotides
        o 1.2  Draft Human Genome
        o 1.2.1  Base Quality Values
        o 1.2.2  ENSEMBL automatic annotation
        o 1.3  Genomes Web Server
        o 1.4  Cross-Reference Information
        o 1.5  Database Files
        o 1.5.1  EST Database Files
        o 1.5.2  GSS Database Files
        o 1.5.3  HUM Database Files
        o 1.5.4  HTG Database Files       
        o 1.6  Sequence Retrieval System (SRS6)
        o 1.7  EMBL Database FAQ
        o 1.8  Disclaimer

        o 2.1 Genome Representation
        o 2.2 New HTC (High Throughput cDNA) division
        o 2.3 EMBL Cumulative Update File 
        o 2.4 Splitting HTG and GSS division files
        o 2.5 Next version of SRS indices 
        o 3.1 Checking Sequence Data For Vector Contamination
        o 3.2 WebIn - WWW Sequence Submission System
        o 3.3 Bulk Submissions
        o 3.4 SEQUIN - Stand-alone Submission Program
        o 3.5 Sequence Alignment Submissions
        o 3.6 Further Submission Information
        o 3.6.1 Annotation Guides


        o 5.1 Electronic Mail Server
        o 5.2 Anonymous FTP Server
        o 5.3 World Wide Web (WWW) Server
        o 5.4 Sequence Similarity Search Servers

        o 6.1 Release 64 Files
        o 6.2 SRS Indices



The EMBL Nucleotide Sequence Database was frozen to make Release 64 on 
02-Sep-2000. The release contains 8,344,436 sequence entries comprising 
9,650,223,037 nucleotides. This represents an increase of about 16% over 
Release 63. A breakdown of Release 64 by division is shown below:

Division               Entries     Nucleotides
----------------- ------------ ---------------
ESTs                 5,565,880   2,194,418,599
Fungi                   41,017      75,333,934
GSSs                 1,717,212     950,099,606
HTG                     77,671   4,263,600,014
Human                  119,154     965,113,287
Invertebrates           54,900     329,846,226
Other Mammals           27,021      25,376,675
Organelles              72,962      61,665,029
Patents                207,677      67,411,887
Bacteriophage            1,595       4,385,850
Plants                  68,956     221,131,770
Prokaryotes             86,977     218,928,626
Rodents                 55,263      92,528,729
STSs                   116,671      51,039,988
Synthetic                3,838       9,763,762
Unclassified             1,174       1,869,994
Viruses                102,523      90,011,114
Other Vertebrates       23,945      27,697,947
                  ------------ ---------------
Total                8,344,436   9,650,223,037

1.1 Nine Billion Nucleotides

On 07-JUL-2000 the number of nucleotides in the EMBL Database has passed the
9,000,000,000 mark. Over the last 12 months (compare Oct 1, 1999: 3.6 Gigabases)
the database size has increased by more than 160%. 

EMBL database statistics are available at URL: 

1.2 Draft Human Genome and HTG division

The completion of the human draft genome sequence has been announced on
26-June-2000. The draft sequence data is available from the EMBL Database 
HTG and HUM divisions.
The total size of the euchromatic portion of the genome is estimated to be 3.2
Gbases. The fact that the total score (FIN + UNFIN) exceeds the size of the 
genome is due to redundancy, the general assumption is that about 30% - 40% of 
the bases are redundant.  
Below are the database statistics for finished and unfinished human sequence
in EMBL database from September 19, 2000. 

------    --------- -----------  -----------
9/2000      910 Mb     3505 Mb      4415 Mb

See also the Genome Monitoring Table for further detailed information  
available from the EBI at URL

1.2.1 Base quality values

Quality scores from draft HTG data are available on the EBI FTP server. The 
gzip'ed files in the directory contain base quality values for unfinished human 
sequences from Japanese, US and European sequencing centres. The FastA-type 
headers contain the EMBL accession number/version of the corresponding database 


>AL009030.9 Phrap Quality (Length:229022, Min: 3, Max: 99)

In order to keep the size of the files within reasonable limits for handling 
purposes, files which in uncompressed form are bigger than 1 Gb, are split 
into smaller files. 

Directory: ftp://ftp.ebi.ac.uk/pub/databases/embl/quality_scores

Current Files:   /htg_sanger1.qscore.gz - /htg_sanger3.qscore.gz
                 /htg_us1.qscore.gz - /htg_us9.qscore.gz

Quality score files are updated on a daily basis.

1.2.2 Ensembl automatic annotation

Ensembl provides automatic annotation to the human draft genome data including 
information on confirmed peptides, confirmed cDNAs and also predicted peptides.
Additionally, repeat prediction along with integration of map information and
SNPs are available.
Updated human genome resources spanning the entire working draft are now 
available. Ensembl has released its automatic annotation for a June 15th 
"frozen" data set at http://freeze.ensembl.org. This URL will now be the stable
location for all subsequent "frozen" dataset updates.
The Ensembl web site is available at URL http://www.ensembl.org/ 
Ensembl is a joint project between the Sanger Centre and EMBL-EBI. 

1.3 Genome WEB Server 

Access to completed genomes
The first completed genomes from viruses, phages and organelles were deposited
into the EMBL Database in the early 1980's. Since then, molecular biology's
shift to obtain the complete sequences of as many genomes as possible combined
with major developments in sequencing technology resulted in hundreds of 
complete genome sequences being added to the database, including Archaea, 
Eubacteria and Eukaryota. Recent additions include Buchnera sp. APS 
(acc# BA000003) and Pseudomonas aeruginosa (acc# AE004091).
EBI's Genome Web Server provides easy access to completed genome sequences and
is available at URL: http://www.ebi.ac.uk/genomes/

Genome Monitoring Table
The Genome MOT presents the status of a number of large eukaryotic genome
sequencing projects. The tables are updated daily and also provide access to
EMBL database entries. The Genome MOTis available at URL:  

1.4 Cross-Reference Information

Links to a growing list of external databases have been expanded allowing 
integration with specialised data collections, such as protein databases, 
species-specific databases, taxonomy databases etc. The WWW-based sequence 
retrieval system (SRS) enable users to easily navigate between cross-referenced 
database entries.

EMBL links to other databases:

Database   Nr of links                                                          
---------- -----------                                                          
RZPD           2002574                                                          
TrEMBL          338688                                                          
Demeter         175252                                                          
SWISS-PROT      143124                                                          
MaizeDB          65929                                                          
FLYBASE          40968                                                          
IMGT/LIGM        37286                                                          
MENDEL           21033                                                          
GDB               8430                                                          
MGD               7998                                                          
TRANSFAC          6620                                                          
SGD               6029                                                          
EPD               3094                                                          
IMGT/HLA          2628                                                          
Total          2859653 

A list of URLs which conform with current DR line references is available:

Demeter     http://ars-genome.cornell.edu
EPD         http://www.epd.isb-sib.ch 
FLYBASE     http://www.fruitfly.org
GDB         http://www.gdb.org 
IMGT/HLA    http://www.ebi.ac.uk/imgt/hla 
IMGT/LIGM   http://imgt.cines.fr:8104 
MGD         http://www.informatics.jax.org 
MaizeDB     http://www.agron.missouri.edu 
MENDEL      http://mbclserver.rutgers.edu/CPGN 
RZPD        http://www.rzpd.de 
SGD         http://genome-www.stanford.edu 
SWISS-PROT  http://www.expasy.ch
TRANSFAC    http://transfac.gbf.de/TRANSFAC 
TrEMBL      http://www.ebi.ac.uk/swissprot/Information/information.html  

1.5 Database Files

In order to keep the size of the data files within reasonable limits for 
handling purposes, additional division files will be added in subsequent
releases as appropriate.

1.5.1 EST Database Files

EST files are now split according to taxonomic subdivisions following the model 
of the taxonomic split of all other EMBL database divisions, e.g. Release 64 
includes files 

est_fun.dat                         Fungi ESTs
est_hum1.dat  -  est_hum23.dat      Human ESTs
est_inv1.dat  -  est_inv4.dat       Invertebrate ESTs
est_mam1.dat  -  est_mam2.dat       Mammal ESTs
est_pln1.dat  -  est_pln8.dat       Plant ESTs
est_pro.dat                         Prokaryote ESTs
est_rod1.dat  -  est_rod19.dat      Rodent ESTs
est_vrt1.dat  -  est_vrt2.dat       Vertebrate ESTs
This should reduce significantly the volume of data  users have to parse in 
order to extract ESTs for specific groups of organisms.  

1.5.2 GSS Database Files

The GSS division has been split into 18 files (gss1.dat-gss18.dat).

1.5.3 HUM Database Files 

The HUM division has been split into 6 files (hum1.dat-hum6.dat).

1.5.4 HTG Database Files

The HTG division has been split into 11 files (htgo.dat and htg1.dat-htg10.dat). 

htgo.dat includes all HTGS_PHASE0 entries. These typically consist of one-to-few
pass reads of a single clone, have not been assembled into contigs and are 
unoriented, unordered, unannotated and contain gaps with runs of 'N's separating
the reads. Low-pass sequence sampling is useful for identifying clones that may 
be gene-rich. Phase0 sequences are used to check whether another center is 
already sequencing this clone. If not, it will be sequenced through phase 1 and
phase 2. When records are updated, the accession numbers will be preserved. 
Files htg1-htg10 include all other HTG entries (HTGS_PHASE1 - HTGS_PHASE2)

1.6 Sequence Retrieval System (SRS6) 

As announced earlier EBI's SRS6 server is available at URL  
http://srs.ebi.ac.uk/ now maps to http://srs6.ebi.ac.uk/.

All external services are available from the Tools button on EBI's Web pages.

If you have any comments and/or suggestions please send these to: 


1.7 EMBL Database FAQ

An EMBL Database FAQ has been created and is available from the EBI at URL 


This document includes information on:

General questions about EMBL and other databases 
Submission procedure 
Updating database entries 
WEBIN-specific questions 
Navigation guide 

1.8 Disclaimer

No guarantee is given as to the completeness and accuracy of the database 
entries, in particular the conformity of sequence data in the database with 
the journal publication where the sequence is also disclosed.


2.1 Genome Representation

At the May 2000 Collaborative Meeting it was confirmed by the sequence database
collaboration DDBJ/EMBL/GenBank to go ahead to transform the currently existing
experimental FTP directory representing genome data into a database division 
CON (Constructed Sequences) to represent complete genomes and other long
sequences constructed from segment entries. The CON division entries will 
contain construct information (accession numbers and sequence locations)
involved in building the genomes. CON entries and according information will
be included into the daily data exchange mechanism between the collaborating 

The CON entry file includes construct information and all accession numbers 
relevant to the genome. Additionally, the complete entry in EMBL format 
(DNA and features) plus the complete DNA sequence in Fasta format is provided.
These entries will be linked, searchable and retrievable through SRS and 
available for BLAST and FASTA homology searching.

For an example representation, see the bacterial genome of Pseudomonas 
aeruginosa (AE004091) in 



2.2 New HTC (High Throughput cDNA) division 

At the May 2000 collaborative meeting DDBJ/EMBL/GenBank agreed to create a new 
database division HTC to represent unfinished High Throughput cDNA sequences. 
HTC sequences may include  5'UTR and 3'UTR regions and (part of a) codin 
region. Upon finishing of these sequences, they will be moved to the 
corresponding taxonomic division. HTC sequence entries will include the keyword
'HTC'. The keyword will be removed once the entry has been included in the 
taxonomic division. 

2.3 EMBL cumulative update file

We intend to discontinue the provision of the single cumulative update file.
Several sites have reported problems handling our EMBL cumulative update file
when it grows beyond 2GB (uncompressed), because of file systems that do not 
support files > 2Gb. Instead of the cumulative.dat.gz file, we will continue to
make available on our FTP server a set of smaller data files, that contain
together the same data as the full cumulative update file, named cum_*.dat.gz 
For further details please check the README file in directory

2.4 Splitting HTG and GSS division files

We plan to split HTG and GSS division files according to taxonomic subdivisions 
following the model of the taxonomic split of all other EMBL database divisions.
This should reduce significantly the volume of data users have to parse in order
to extract HTGs and GSSs for specific groups of organisms. Files will be named 
accordingly e.g. 
HTGS_PHASE0 sequences will be included in files htgo_hum.dat, htgo_inv.dat
htgo_rod.dat etc, while htgo.dat will include all remaining HTGS_PHASE0 entries.
HTGS_PHASE1 - HTGS_PHASE2 sequences will be included in files htg_hum.dat, 
htg_inv.dat, htg_rod.dat etc while htg.dat will include all remaining HTG 
GSS sequences will be included in files gss_fun.dat, gss_hum.dat etc, while 
gss.dat will include all remaining GSS entries. 

2.5 Next version of SRS indices

Please note that the next version of SRS indices will be for version 607x and not 606.

3.1 Checking Sequence Data For Vector Contamination

We urge submitters to remove vector contamination from sequence data before 
submitting to the database. To assist submitters the EBI is providing a Vector
Screening Service using the latest implementation of the BLAST algorithm and a
special sequence databank known as EMVEC. EMVEC is an extraction of sequences 
from  the SYNthetic division of EMBL containing more than 2000 sequences
commonly used in cloning and sequencing experiments. EMVEC is by no means a 
complete vector databank but EBI believes it is representative of the kind of 
material used in modern sequencing and should be useful to submitters. The 
databank will be updated with each release of EMBL and made publicly available
on the EBI's ftp server for those who wish to have it.

The interactive WWW service can be found at:


The results will list sequences producing significant alignments and associated
information like vector name, score, alignment etc

3.2 WebIn - WWW Sequence Submission System

WebIn is the preferred WWW Sequence Submission System for submitting nucleotide
sequence data and associated biological information to the EMBL Nucleotide 
Sequence Database at the European Bioinformatics Institute(EBI). To access WebIn
at the EBI please use the following URL:


Database entries submitted to the EMBL Nucleotide Sequence Database at the EBI
will be exchanged and shared among the International Collaboration of Nucleotide
Sequence Databases (DDBJ/EMBL/GenBank).

WebIn guides the user through a sequence of WWW forms allowing the submission 
of sequence data and descriptive information in an interactive and easy way.
All the information required to create a database entry will be collected 
during this process:

1 Submitter Information
2 Release Date Information
3 Sequence Data, Description and Source Information
4 Reference Citation Information
5 Feature Information (e.g. coding regions, regulators,
 signals etc.)

EBI staff will process data submissions within 2 working days and send the 
database accession number(s) assigned to your data to your e-mail address.

3.3 Bulk Submissions

With the aim to make bulk sequence submission less time consuming for the 
submitters, a new web-based submission system can now be accessed from the
WebIn page. Authors planning to submit a large number of similar sequences
(i.e.,>25) are presented with an option for "Bulk WebIn Submission". When 
choosing thebulk path, submitters carry on the usual WebIn submission procedure
untilhaving finished a first and single representative sequence. During the 
submission process database staff will interactively assist in making the 
submission of this specific data as convenient as possible, thus saving the 
author the time and effort required to complete numerous submission  events 
Alternatively, authors planning to submit very large numbers of similar 
sequences should contact the database before submitting the data. Database
staff will create series of templates and communicate these to the author for 
completion with just the information unique to each sequence required. 
Please contact database staff if you require further information.

e-mail: datasubs@ebi.ac.uk

Tel:   +44-1223-494499
Fax:   +44-1223-494472

3.4 SEQUIN - Stand-alone Submission Program

Sequin is the multi-platform (Mac/PC/Unix) stand-alone software tool developed
by the NCBI for submitting entries to the EMBL, GenBank, or DDBJ sequence
databases. The Sequin program, along with detailed downloading and installation
instructions plus general information are available from the EBI via WWW and
anonymous FTP.


3.5 Sequence Alignment Submissions

The EBI accepts submissions of alignment data (e.g. from phylogenetic and 
population analysis etc) of both nucleotide or amino-acid sequences, database
staff assigns an alignment number (e.g. ds38200), which is then communicated to
the submitter. We suggest that this number is quoted in the resulting 
Alignment data and associated information are made available via EBI's network 
servers (see below).


As well as your alignment data we require information describing your alignment
(see table below) Please provide information for all fields.

 Description Field      Information required

 TITLE:                 Title of alignment
 SUBMITTER:             Name, Affiliation, Phone, Fax, Email

 RELEASE DATE:          Public Immediately / if Confidential please
                        provide hold date

 CITATION:              If known please provide complete Author list,
                        Title, Journal, Year of publication, Page 
 ALIGNMENT METHOD:      Method of alignment and format submitted,
                        parameters of alignment sequences used (if

 DESCRIPTION OF         e.g. Gaps indicated by a dash '-'
 DESCRIPTION OF         Describe sequences aligned, including accession
 ALIGNMENT:             numbers (if known) and abbreviation of clones or
                        taxon used in alignment file. If your alignment
                        contains sequences derived from multiple
                        taxoonomic sources, please provide the full name
                        of each organism


CLUSTALW etc) or Sequin output.

A sample alignment in NEXUS format can be viewed at

NOTE 1: Alignments can be created within Sequin or imported into Sequin from 
files in a standard alignment format like NEXUS or PHYLIP.

NOTE 2: If reporting new primary sequence data, we suggest that you submit
the complete individual sequence files (e.g. via Sequin or WebIn), in order to
include the sequence data as individual entries in the EMBL database. If gaps
have been introduced for the alignment, please leave them out when sending the
individual sequence files.

SENDING ALIGNMENT DATA to the EMBL Nucleotide Sequence Database
Sequence alignment data can be sent to the Nucleotide Sequence Database by 
Electronic mail to datasubs@ebi.ac.uk

Alignment data and additional information are available via the EBI servers:

EBI WWW server:

EBI FTP server:  by anonymous FTP from FTP.EBI.AC.UK in directory

EBI File server: by sending an e-mail message to netserv@ebi.ac.uk
                 including the line HELP ALIGN or GET ALIGN:DS8200.DAT

3.6 Further Submission Information

3.6.1 Annotation Guides

To help and guide submitters in annotating their sequences, two online guides 
are available via hyperlinks  from within WebIn: 
EMBL Annotation Examples (http://www3.ebi.ac.uk/Services/Standards/web/) and 
EMBL Features and Qualifiers (http://www3.ebi.ac.uk/Services/WebFeat/). The 
annotation examples consist of a list of EMBL approved feature table
annotations for common biological sequences. The EMBL Features and Qualifiers
is a complete list of feature table key and qualifier definitions providing 
detailed descriptions, mandatory and optional qualifiers and usage examples.

For further information on submission of sequence data to the EMBL Nucleotide 
Sequence Database please access:


or contact database staff at:

EMBL Nucleotide Sequence Submissions
e-mail: datasubs@ebi.ac.uk
telephone: +44-1223-494499
telefax: +44-1223-494472


We encourage authors to include a reference to the EMBL Database in 
publications related to their research.

When citing data in the EMBL Database, we suggest to give the according 
primary accession  and the publication in which the sequence first appeared. 
For unpublished data, we suggest to contact the original submitters for 
recent publication information or revisions of the data.

We suggest to also provide a reference for the EMBL Database itself. Our 
recent publication describing the EMBL database should be cited:

Baker W., van den Broek, A., Camon E., Hingamp P., Sterk P., Stoesser G.,
and Tuli M.A.. 'The EMBL Nucleotide Sequence Database', 
Nucl. Acids Res., 28 (1), 19-23 (2000). 
Example: The numbers in parentheses refer to the REFERENCE in the EMBL
database entry, and to the EMBL citation above.

"Sequence entry X56734 (1) has been retrieved from the EMBL Database (2)
and showed significant sequence similarity to ..."

(1) Oxtoby, E., et al., Plant Mol. Biol. 17:209-219(1991).
(2) Baker, W., et al., Nucl. Acids Res. 28:19-23(2000)


5.1 Electronic Mail Server

Computer users with access to Internet (directly or via a gateway) can obtain 
copies of database entries, documentation or the data submission form, by 
sending commands to a file server running at EBI. New and updated EMBL 
nucleotide sequence entries are made available on the server on a daily basis.

To use this facility, send file server commands (as electronic mail)  to the 
address netserv@ebi.ac.uk. Each line of the mail message should consist of a 
single file server request.

The most important file server request, to get started, is:


If the file server receives this command, it will return a helpfile to the 
sender, explaining in some detail how to use the facility. For example, to 
request a copy of the nucleotide sequence with accession number X55652, use 
the command:

GET NUC:X55652

The file server offers various other services, (eg., access to nucleotide and 
protein sequence data, protein structure data, software), details of which are
provided in the HELP file.

5.2 Anonymous FTP Server

An alternative method of accessing the EBI archives is to use the Internet 
File transfer protocol (ftp). Researchers with direct access to the Internet
can use the FTP program on their local machine to connect to the host
FTP.EBI.AC.UK and enter the username "anonymous" and their email address as
The directory pub/help contains detailed information about the data available
from the EBI  anonymous FTP server which includes the complete EMBL Nucleotide
Sequence Database releases as well as daily and weekly updates and a cumulative
update file (in UNIX-compressed format)in the following directories:

EMBL quarterly release:   pub/databases/embl/release
EMBL updates:   pub/databases/embl/new

5.3 World Wide Web (WWW) Server

The EBI operates a WWW server with URL http://www.ebi.ac.uk/ which gives access
to information about the EBI and it's products and services. Nucleotide 
sequences can be retrieved by a simple query by accession number, or more 
complex queries can be contructed using an SRS WWW databank browser. Nucleotide
sequences can also be submitted to the database using the interactive submission
system WebIn at URL:


5.4 Sequence Similarity Search Servers

The EBI offers two network servers for sequence similarity searches via 
electronic mail or interactive WWW forms:
FASTA based on W. Pearson's FASTA algorithm. Allows local similarity
      searches of protein and nucleotide sequence databases. 
      Send "help" to Fasta@EBI.AC.UK  or use 
      URL http://www.ebi.ac.uk/fasta3/

BLAST based on the NCBI and WU-Blast software Send "help" to
      Blast@EBI.AC.UK  or use URL http://www.ebi.ac.uk/blast2/
BLITZ allows very fast searches of protein sequence databases for
      local similarities using an exhaustive Smith-Waterman matching
      algorithm. Compugen's BIC_SW software is running on a 
      Biocellerator (BIC-2) Send "help" to Blitz@EBI.AC.UK  or 
      use URL http://www.ebi.ac.uk/bic_sw/


6.1 Release 64 Files

The release contains the files shown below, in the order listed. File sizes are 
given as numbers of records.

File Number File Name     Description                    Number of Records

 1          DELETEAC.TXT  Deleted accession numbers                  44649
 2          FTABLE.TXT    Feature Table Documentation                  465
 3          RELNOTES.TXT  Release Notes (this document)                915
 4          SUBFORM.TXT   Data Submission Form                         418
 5          SUBINFO.TXT   Data Submission Documentation                333
 6          UPDATE.TXT    Data Update Form                             107
 7          USRMAN.TXT    User Manual                                 1469
 8          ACNUMBER.NDX  Accession Number Index                   8372365
 9          CITATION.NDX  Citation Index                           1872434
 10         DIVISION.NDX  Division Index                                23
 11         KEYWORD.NDX   Keyword Index                            3109242
 12         SHORTDIR.NDX  Short Directory Index                   21428207
 13         SPECIES.NDX   Species Index                            2888410
 14         EST_FUN.DAT   EST Sequences                            3491596
 15         EST_HUM1.DAT  EST Sequences                            7242162
 16         EST_HUM2.DAT  EST Sequences                            7383411
 17         EST_HUM3.DAT  EST Sequences                            7092087
 18         EST_HUM4.DAT  EST Sequences                            6958043
 19         EST_HUM5.DAT  EST Sequences                            7086795
 20         EST_HUM6.DAT  EST Sequences                            7098043
 21         EST_HUM7.DAT  EST Sequences                            7136249
 22         EST_HUM8.DAT  EST Sequences                            7031857
 23         EST_HUM9.DAT  EST Sequences                            7156374
 24         EST_HUM10.DAT EST Sequences                            6859020
 25         EST_HUM11.DAT EST Sequences                            6661083
 26         EST_HUM12.DAT EST Sequences                            6431484
 27         EST_HUM13.DAT EST Sequences                            6811351
 28         EST_HUM14.DAT EST Sequences                            6856402
 29         EST_HUM15.DAT EST Sequences                            7036586
 30         EST_HUM16.DAT EST Sequences                            7306475
 31         EST_HUM17.DAT EST Sequences                            7263236
 32         EST_HUM18.DAT EST Sequences                            7357458
 33         EST_HUM19.DAT EST Sequences                            7444208
 34         EST_HUM20.DAT EST Sequences                            7476190
 35         EST_HUM21.DAT EST Sequences                            6699624
 36         EST_HUM22.DAT EST Sequences                            6963358
 37         EST_HUM23.DAT EST Sequences                            4588499
 38         EST_INV1.DAT  EST Sequences                            6431773
 39         EST_INV2.DAT  EST Sequences                            6042873
 40         EST_INV3.DAT  EST Sequences                            6293598
 41         EST_INV4.DAT  EST Sequences                            4046341
 42         EST_MAM1.DAT  EST Sequences                            6114230
 43         EST_MAM2.DAT  EST Sequences                            2356039
 44         EST_PLN1.DAT  EST Sequences                            6750911
 45         EST_PLN2.DAT  EST Sequences                            6219344
 46         EST_PLN3.DAT  EST Sequences                            5830564
 47         EST_PLN4.DAT  EST Sequences                            7215994
 48         EST_PLN5.DAT  EST Sequences                            7046836
 49         EST_PLN6.DAT  EST Sequences                            6762278
 50         EST_PLN7.DAT  EST Sequences                            6720107
 51         EST_PLN8.DAT  EST Sequences                            6029205
 52         EST_PRO.DAT   EST Sequences                              38548
 53         EST_ROD1.DAT  EST Sequences                            7331559
 54         EST_ROD2.DAT  EST Sequences                            7567611
 55         EST_ROD3.DAT  EST Sequences                            7220551
 56         EST_ROD4.DAT  EST Sequences                            7549688
 57         EST_ROD5.DAT  EST Sequences                            6811012
 58         EST_ROD6.DAT  EST Sequences                            7086810
 59         EST_ROD7.DAT  EST Sequences                            9771985
 60         EST_ROD8.DAT  EST Sequences                            9130283
 61         EST_ROD9.DAT  EST Sequences                            7665029
 62         EST_ROD10.DAT EST Sequences                            9177208
 63         EST_ROD11.DAT EST Sequences                            9743196
 64         EST_ROD12.DAT EST Sequences                            9700691
 65         EST_ROD13.DAT EST Sequences                            9653685
 66         EST_ROD14.DAT EST Sequences                            9473210
 67         EST_ROD15.DAT EST Sequences                            9015774
 68         EST_ROD16.DAT EST Sequences                            6666497
 69         EST_ROD17.DAT EST Sequences                            7649778
 70         EST_ROD18.DAT EST Sequences                            7420422
 71         EST_ROD19.DAT EST Sequences                             738690
 72         EST_VRT1.DAT  EST Sequences                            7641169
 73         EST_VRT2.DAT  EST Sequences                            2254064
 74         FUN.DAT       Fungi Sequences                          3736027
 75         GSS1.DAT      Genome Survey Sequences                  6116578
 76         GSS2.DAT      Genome Survey Sequences                  6118824
 77         GSS3.DAT      Genome Survey Sequences                  6268149
 78         GSS4.DAT      Genome Survey Sequences                  6628318
 79         GSS5.DAT      Genome Survey Sequences                  6554451
 80         GSS6.DAT      Genome Survey Sequences                  6616068
 81         GSS7.DAT      Genome Survey Sequences                  6639716
 82         GSS8.DAT      Genome Survey Sequences                  6644800
 83         GSS9.DAT      Genome Survey Sequences                  6958158
 84         GSS10.DAT     Genome Survey Sequences                  6788195
 85         GSS11.DAT     Genome Survey Sequences                  7155659
 86         GSS12.DAT     Genome Survey Sequences                  6988978
 87         GSS13.DAT     Genome Survey Sequences                  6978243
 88         GSS14.DAT     Genome Survey Sequences                  6402203
 89         GSS15.DAT     Genome Survey Sequences                  6646868
 90         GSS16.DAT     Genome Survey Sequences                  7448747
 91         GSS17.DAT     Genome Survey Sequences                  6669805
 92         GSS18.DAT     Genome Survey Sequences                  1027489
 93         HTG1.DAT      High Throughput Genome Sequences         7854248
 94         HTG2.DAT      High Throughput Genome Sequences         5995734
 95         HTG3.DAT      High Throughput Genome Sequences         4210260
 96         HTG4.DAT      High Throughput Genome Sequences         4724917
 97         HTG5.DAT      High Throughput Genome Sequences         8718298
 98         HTG6.DAT      High Throughput Genome Sequences         8721834
 99         HTG7.DAT      High Throughput Genome Sequences         8979368
 100        HTG8.DAT      High Throughput Genome Sequences         8137472
 101        HTG9.DAT      High Throughput Genome Sequences         7846179
 102        HTG10.DAT     High Throughput Genome Sequences         4273070
 103        HTGO.dat      High Throughput Genome Sequences         8701440
 104        HUM1.DAT      Human Sequences                          9494007
 105        HUM2.DAT      Human Sequences                          5320579
 106        HUM3.DAT      Human Sequences                          3561983
 107        HUM4.DAT      Human Sequences                          2858503
 108        HUM5.DAT      Human Sequences                          2298449
 109        HUM6.DAT      Human Sequences                          1644433
 110        INV.DAT       Invertebrate Sequences                   9495348
 111        MAM.DAT       Other Mammal Sequences                   1908267
 112        ORG.DAT       Organelle Sequences                      5140625
 113        PATENT.DAT    Patent Sequences                         8110279
 114        PHG.DAT       Bacteriophage Sequences                   217840
 115        PLN.DAT       Plant Sequences                          8269953
 116        PRO1.DAT      Prokaryote Sequences                     6104496
 117        PRO2.DAT      Prokaryote Sequences                     4233076
 118        ROD.DAT       Rodent Sequences                         4755562
 119        STS.DAT       STS Sequences                            7970081
 120        SYN.DAT       Synthetic Sequences                       394629
 121        UNC.DAT       Unclassified Sequences                    106371
 122        VRL.DAT       Viral Sequences                          7545287
 123        VRT.DAT       Other Vertebrate Sequences               1787491

6.2 SRS Indices

SRS indices can be found on the FTP server in the srs directory 
See README file for details. 
Please note that the next version of SRS indices will be for version 607x
and not 606.



The following table shows the growth of the EMBL Nucleotide Sequence 
Database at each release.

 Release Month        Entries      Nucleotides

 1       06/1982          568           585433
 2       04/1983          811          1114447
 3       12/1983         1481          1654863
 4       08/1984         1698          2147205
 5       04/1985         2378          2874493
 6       08/1985         4835          4567592
 7       12/1985         5789          5622638
 8       04/1986         6395          6353040
 9       09/1986         7630          7813214
 10      12/1986         8817          9766948
 11      04/1987        11621         12189783
 12      07/1987        12706         13638061
 13      10/1987        14397         16023478
 14      01/1988        15344         17272160
 15      05/1988        17961         20318442
 16      08/1988        19592         22625941
 17      11/1988        20695         24211054
 18      02/1989        22938         27249830
 19      05/1989        24365         29066676
 20      08/1989        26223         31240948
 21      11/1989        28679         34748087
 22      02/1990        31508         38165786
 23      05/1990        34902         42923803
 24      08/1990        37784         47354438
 25      11/1990        41580         52900354
 26      02/1991        43745         55859549
 27      05/1991        46871         59915244
 28      09/1991        54558         70448052
 29      12/1991        57655         75400487
 30      03/1992        63378         83574342
 31      06/1992        72481         94390065
 32      09/1992        79377        101292310
 33      12/1992        89100        111413979
 34      03/1993        99591        121420828
 35      06/1993       108973        131880111
 36      09/1993       127933        145401156
 37      12/1993       146576        158171400
 38      03/1994       167777        177550115
 39      06/1994       182615        192195819
 40      09/1994       209352        211017104
 41      12/1994       230950        226259607
 42      03/1995       303206        262559786
 43      06/1995       420111        315840053
 44      09/1995       506190        363273777
 45      12/1995       622566        427620278
 46      03/1996       701246        473691480
 47      06/1996       827174        550739395
 48      09/1996       928067        608931850
 49      12/1996      1047263        696183789
 50      03/1997      1187455        789755858
 51      06/1997      1432941        931351601
 52      10/1997      1787004       1181167498
 53      12/1997      1917868       1281391651
 54      03/1998      2125225       1427634373
 55      06/1998      2330040       1607673907
 56      09/1998      2689618       1904091473
 57      12/1998      3046471       2164718256
 58      03/1999      3272064       2355200790
 59      06/1999      3952878       2924568545
 60      09/1999      4719266       3543553093
 61      12/1999      5303436       4508169737
 62      03/2000      5865742       6120908677
 63      06/2000      6760113       8255674441
 64      09/2000      8344436       9650223037